Western Blot Analysis of Antioxidant Proteins

Anti-Nrf2 (ab62352), anti-Ubiquitin (ab134952), anti-IL-1β (ab45692), anti–NF–κB p65 (ab16502), anti-SOD1 (ab13498), anti-GPx1 (ab108427) and anti-catalase (ab76110) antibodies were purchased from Abcam Technology (Abcam Technology, England). Anti-phospho–NF–κB p65 antibodies (CST #3031), anti-phospho-IκBα (CST #2859) and anti-phospho-IKKβ (CST #2694) antibodies were purchased from Cell Signalling Technology (Cell Signalling Technology, USA). Anti–HO–1 (SC-136960) and anti-NQO1 (SC-271116) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-β-actin (60008-1-lg), anti-Histone-H3 (17168-1-AP) and anti-GCLM (14241-1-AP) antibodies were purchased from Proteintech Group (Proteintech Group, USA). Isolation of cell fractions and Western blotting were performed as detailed previously [36 (link)].