Drosophila Neurodegeneration Research Protocols

Flies were maintained in standard cornmeal media at 25°C. Transgenic fly lines carrying UAS-Luciferase RNAi, UAS-human tau and UAS-Aβ42 were previously described [43 (link),63 (link)]. Eaat1^SM2 and a precise excision line Eaat1^PE (used as a control line for Eaat1^SM2) are kind gifts from Dr. D. J. van Meyel (McGill University) [55 (link)]. The elav-GAL4 (#458), Repo-GAL4 (#7415), GMR-GAL4 (#1104), UAS-wfs1 RNAi^TRiP (#53330), UAS-mcherry RNAi (#35785), wfs1^e03461 (#18157), wfs1^MI14041 (#59250), UAS-wfs1 (#8356), UAS-wfs1 (#8357), UAS-mitoGFP (#8442) and UAS-Luciferase (#35788), Opa1^S3475 (#12188) were obtained from the Bloomington Stock Center. UAS-wfs1 RNAi^GD (#42664) was obtained from the Vienna Drosophila RNAi Center. wfs1^LL07290 (#142011) was obtained from Kyoto Stock Center. Genotypes and ages of all flies used in this study are provided in S1 Table. Experiments were performed using age-matched male or female flies.

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Sakakibara Y., Sekiya M., Fujisaki N., Quan X, & Iijima K.M. (2018). Knockdown of wfs1, a fly homolog of Wolfram syndrome 1, in the nervous system increases susceptibility to age- and stress-induced neuronal dysfunction and degeneration in Drosophila. PLoS Genetics, 14(1), e1007196.

Publication 2018

elav-GAL4 Repo-GAL4 GMR-GAL4 UAS-mcherry RNAi UAS-wfs1 UAS-mitoGFP UAS-Luciferase

Drosophila Female Flies Genotypes Gifts Human Luciferase Male Rnai Transgenic Wfs1

Corresponding Organization : Nagoya City University

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Variable analysis

independent variables

Transgenic fly lines carrying UAS-Luciferase RNAi, UAS-human tau, and UAS-Aβ42
Eaat1SM2 and a precise excision line Eaat1PE (used as a control line for Eaat1SM2)
UAS-wfs1 RNAi (TRiP and GD)
UAS-mcherry RNAi
Wfs1e03461 and wfs1MI14041
UAS-wfs1 (two lines)
UAS-mitoGFP
UAS-Luciferase
Opa1S3475

dependent variables

Not explicitly mentioned

control variables

Flies were maintained in standard cornmeal media at 25°C
Eaat1PE (precise excision line) used as a control line for Eaat1SM2

positive controls

UAS-Luciferase

negative controls

Eaat1PE (precise excision line) used as a control line for Eaat1SM2
UAS-mcherry RNAi

Annotations

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