Chondrogenic Differentiation of Human MSCs

Human iliac crest-derived mesenchymal stem cells (MSCs) from three donors aged 22–24 years (Lonza Biosciences) were subjected to Transwell chondrogenic differentiation as described by Murdoch et al. [34 (link)] and Barter et al. [35 (link)]. Cartilage discs were harvested after 3, 7 and 14 days and RNA extracted from the ground tissue using the Trizol/chloroform method (Invitrogen). cDNA was synthesised as for cartilage samples and subjected to qRT-PCR, as above.

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Reynard L.N., Ratnayake M., Santibanez-Koref M, & Loughlin J. (2016). Functional Characterization of the Osteoarthritis Susceptibility Mapping to CHST11—A Bioinformatics and Molecular Study. PLoS ONE, 11(7), e0159024.

Publication 2016

Trizol/chloroform method

Cartilage Cdna Chloroform Chondrogenic Donors Human Iliac crest Mesenchymal stem cells Tissue Trizol

Corresponding Organization : Newcastle University

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Variable analysis

independent variables

Duration of chondrogenic differentiation (3 days, 7 days, 14 days)

dependent variables

Gene expression of cartilage-related markers (not explicitly mentioned)

control variables

Human iliac crest-derived mesenchymal stem cells (MSCs) from three donors aged 22–24 years
Transwell chondrogenic differentiation protocol
RNA extraction using Trizol/chloroform method
CDNA synthesis and qRT-PCR analysis

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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