Harvested tumor grafts were fixed in 10% zinc-formalin (Fisher Scientific) at room temperature overnight, then transferred to 70% ethanol for paraffin embedding. Immunohistochemical staining for Ki67 and cleaved caspase-3 was performed on serial sections. Whole slide digital imaging was analyzed using QuPath software for positive cell counts, using sigma level 2.0 and threshold level 0.3 [17 (link)].
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