Hypoxic Culture of Human Adipose-Derived Stem Cells

hADSCs were purchased (Lonza, Basel, Switzerland) and cultured in Dulbecco’s Modified Eagle Medium (Gibco BRL, Gaithersburg, MD, USA) supplemented with 100 units/mL of penicillin (Gibco BRL), 10% (v/v) fetal bovine serum (FBS, Gibco BRL), and 100 μg/mL of streptomycin (Gibco BRL). All experiments were performed using hADSCs within five passages. To induce hypoxic condition in vitro, hADSCs were cultured under 1% oxygen and serum-free medium, as previously described [28 (link)].

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Lee T.J., Shim M.S., Yu T., Choi K., Kim D.I., Lee S.H, & Bhang S.H. (2018). Bioreducible Polymer Micelles Based on Acid-Degradable Poly(ethylene glycol)-poly(amino ketal) Enhance the Stromal Cell-Derived Factor-1α Gene Transfection Efficacy and Therapeutic Angiogenesis of Human Adipose-Derived Stem Cells. International Journal of Molecular Sciences, 19(2), 529.

Publication 2018

hADSCs Dulbecco’s Modified Eagle Medium penicillin FBS streptomycin

Eagle Hypoxic Oxygen Penicillin Serum Streptomycin

Corresponding Organization : CHA University

Other organizations : Incheon National University, Kyung Hee University, Washington University in St. Louis, Samsung Medical Center

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Variable analysis

independent variables

Oxygen concentration (1% oxygen)

dependent variables

Not explicitly mentioned

control variables

Cell culture medium (Dulbecco's Modified Eagle Medium)
Serum concentration (10% fetal bovine serum)
Antibiotics (100 units/mL of penicillin, 100 μg/mL of streptomycin)
Cell passage number (within five passages)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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