Senescence-Associated Secretory Phenotype Factors

SASP factors: CCL‐2/MCP‐1, IL1α, MMP‐1, PAI1, TNFα, IL‐6, and IL‐10 (Basisty et al., 2020 (link); Camell et al., 2021 (link); Englund et al., 2021 (link); Huang et al., 2018 (link)). CCL‐2/MCP‐1, IL1α, MMP‐1, PAI1, and cystatin C were quantified using multiplex magnetic bead immunoassays (R&D Systems) based on Luminex xMAP multianalyte profiling platform (Luminex® 100/200™ System) according to the manufacturer's protocol and analyzed on Bio‐Rad Bio‐Plex Manager 6.1. Cytokine biomarkers analyzed included C‐reactive protein (CRP), tumor necrosis factor alpha (TNFα), and its receptors (TNFα‐R1 and TNFα‐R2), interleukin 6 soluble receptor (sIL‐6R), and interleukin 10 (IL‐10) as previously described (Langmann et al., 2017 (link)). For all proteins, more than 80% of the samples were within the detectable range. Undetectable targets were replaced with the lowest value for each protein.

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Hamsanathan S., Anthonymuthu T., Prosser D., Lokshin A., Greenspan S.L., Resnick N.M., Perera S., Okawa S., Narasimhan G, & Gurkar A.U. (2024). A molecular index for biological age identified from the metabolome and senescence‐associated secretome in humans. Aging Cell, 23(4), e14104.

Publication 2024

multiplex magnetic bead immunoassays Bio‐Plex Manager 6.1

Corresponding Organization :

Other organizations : University of Pittsburgh, UPMC Hillman Cancer Center, University of Pittsburgh Medical Center, McGowan Institute for Regenerative Medicine, Florida International University

Top 5 similar protocols

Variable analysis

independent variables

CCL‐2/MCP‐1
IL1α
MMP‐1
TNFα
IL‐6
IL‐10

dependent variables

CCL‐2/MCP‐1
IL1α
MMP‐1
TNFα
IL‐6
IL‐10
C‐reactive protein (CRP)
Tumor necrosis factor alpha (TNFα)
TNFα‐R1
TNFα‐R2
Interleukin 6 soluble receptor (sIL‐6R)
Interleukin 10 (IL‐10)

control variables

Luminex® 100/200™ System
Bio‐Rad Bio‐Plex Manager 6.1

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