The cytogenetic analysis from bone marrow cells was performed by G-banding as previously described by De Souza et al., 2014.10 (link) Chromosomes were identified and arranged according to the International System for Cytogenomic Nomenclature, 2020.11 (link) Fluorescence in situ hybridization (FISH) analyses were done to confirm the chromosome alterations using the following probes: D7S486 spectrum orange/CEP7 spectrum Green, LSI MLL dual-color break-apart rearrangement probe, LSI p53 spectrum orange, LSI EGR1 Spectrum Orange/ LSI D5S23, D5S721 Spectrum Green, LSI PML Spectrum Orange/ LSI RARA Spectrum Green and LSI RUNX1T1 Spectrum Orange Probe/ RUNX1 Spectrum Green Probe. The probes were from Vysis, Abbott Laboratories, USA. The slides preparation was done according to manufacturer protocols.
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Cytogenetic Analysis of Bone Marrow Cells
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Other organizations : Instituto Nacional do Câncer, Hospital Universitário Oswaldo Cruz, Universidade Federal do Rio de Janeiro
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Variable analysis
independent variables
- Cytogenetic analysis by G-banding
- Fluorescence in situ hybridization (FISH) analyses
- Identification and arrangement of chromosomes
- Confirmation of chromosome alterations using FISH probes
- Cytogenetic analysis method described by De Souza et al., 2014
- Chromosome identification and arrangement according to the International System for Cytogenomic Nomenclature, 2020
- FISH probes used (D7S486 spectrum orange/CEP7 spectrum Green, LSI MLL dual-color break-apart rearrangement probe, LSI p53 spectrum orange, LSI EGR1 Spectrum Orange/ LSI D5S23, D5S721 Spectrum Green, LSI PML Spectrum Orange/ LSI RARA Spectrum Green and LSI RUNX1T1 Spectrum Orange Probe/ RUNX1 Spectrum Green Probe)
- Slide preparation according to manufacturer protocols
- No positive or negative controls were explicitly mentioned.
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