Glioblastoma and Monocyte Cell Culture

U87-MG (ATCC HTB-14), U251 (Sigma-Aldrich), and LN229 (ATCC CRL2611) human glioblastoma cells and THP1 human monocytes (ATCC TIB-202) were passaged fewer than six months and verified by providing companies using short tandem repeat (STR) profiling and confirmed to be Mycoplasma free. Cells for culture were obtained from U87-Bev^R, U87-Bev^S, SF7300, and SF7796 xenografts generated as described^{3 (link), 19 (link)}. Glioblastoma cells were cultured in DMEM High Glucose/F-12 50/50% mixture media with 10% FBS and 1% penicillin/streptomycin at 37°C. THP1 cells were cultured in RPMI-1640 medium with 10% FBS, L-glutamine (2 mmol/L), 2-mercaptoethanol (55 µM; Gibco), and 1% penicillin/streptomycin at 37°C.

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Castro B.A., Flanigan P., Jahangiri A., Hoffman D., Chen W., Kuang R., De Lay M., Yagnik G., Wagner J.R., Mascharak S., Sidorov M., Shrivastav S., Kohanbash G., Okada H, & Aghi M.K. (2017). Macrophage migration inhibitory factor downregulation: a novel mechanism of resistance to anti-angiogenic therapy. Oncogene, 36(26), 3749-3759.

Publication 2016

LN229 L-glutamine 2-mercaptoethanol

2 mercaptoethanol Cells Cells culture Cultured medium Glioblastoma Glucose Human L glutamine Monocytes Mycoplasma Penicillin Short tandem repeat Streptomycin Xenografts

Corresponding Organization :

Other organizations : Neurological Surgery, University of California, San Francisco

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Variable analysis

independent variables

Cell lines used: U87-MG (ATCC HTB-14), U251 (Sigma-Aldrich), LN229 (ATCC CRL2611) human glioblastoma cells, and THP1 human monocytes (ATCC TIB-202)

dependent variables

Not explicitly mentioned

control variables

Cells were passaged fewer than six months and verified by providing companies using short tandem repeat (STR) profiling
Cells were confirmed to be Mycoplasma free
Glioblastoma cells were cultured in DMEM High Glucose/F-12 50/50% mixture media with 10% FBS and 1% penicillin/streptomycin at 37°C
THP1 cells were cultured in RPMI-1640 medium with 10% FBS, L-glutamine (2 mmol/L), 2-mercaptoethanol (55 µM; Gibco), and 1% penicillin/streptomycin at 37°C

controls

Cells for culture were obtained from U87-Bev^R, U87-Bev^S, SF7300, and SF7796 xenografts generated as described in references 3 and 19, which likely serve as positive and negative controls for the study

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