AMPK and Notch Modulation in gADSC Adipogenesis

gADSCs were seeded and cultured in 6-well plates. When the cell confluence reached 50%, cells were treated with 0.2 mM AICAR (AMPK activator), 4.0 μM Dorsomorphin (Compound C) 2HCl (AMPK inhibitor), 0.4 mM Valproic acid (VPA) (NOTCH activator), and 1.2 μM LY411575 (NOTCH inhibitor) for 24 h. Untreated gADSCs and gADSCs treated with AICAR, Dorsomorphin (Compound C) 2HCl, Valproic acid (VPA), and LY411575 were simultaneously differentiated into adipocytes. The induction and identification were conducted using a method similar to that of the induction of gADSCs differentiation into adipocytes in this study.

Free full text: Click here

Wang Z., Bi M., Zhe X., Wang X., Dai B., Han X., Ren B., Liang H, & Liu D. (2024). Molecular mechanism underlying miR-204-5p regulation of adipose-derived stem cells differentiation into cells from three germ layers. Cell Death Discovery, 10, 95.

Publication 2024

Corresponding Organization :

Other organizations : Inner Mongolia University

Top 5 similar protocols

Variable analysis

independent variables

AICAR (AMPK activator)
Dorsomorphin (Compound C) 2HCl (AMPK inhibitor)
Valproic acid (VPA) (NOTCH activator)
LY411575 (NOTCH inhibitor)

dependent variables

Adipocyte differentiation of gADSCs

control variables

Cell confluence at 50%
Treatment duration of 24 h

controls

Untreated gADSCs (Negative control)
GADSCs treated with AICAR, Dorsomorphin (Compound C) 2HCl, Valproic acid (VPA), and LY411575 (Experimental conditions)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!