Quantification of Metabolic Enzymes via Western Blot

Western blotting was conducted as previously described ^{49 , 50 (link)}. Tumor tissues were powdered in a liquid nitrogen-cooled mortar and pestle and then lysed in 10 volumes of NP40 buffer as previously described ^{51 (link)–53 (link)}. The primary antibodies and concentrations used for Western blotting were following: G6PD (1:1,000, Cell Signaling Technology, #12263S), Vinculin (1:5,000, Sigma, #V4505), Myc tag antibody(1:2,000, Cell Signaling Technology, #2276S), TKT (1:1,000, Cell Signaling Technology, #8616S), PGD (1:1,000, Cell Signaling Technology, #13389S), SLC7A11 (1:2,000, Cell Signaling Technology, #12691S), GLUT1 (1:1,000, Thermo fisher, #PA5–16793), GLUT3 (1:1,000, santa cruz, #sc-74497), NRF2 (1:1,000, Cell Signaling Technology, # 12721S).