Escherichia
coli Stellar (Clontech Laboratories, Mountain View, CA) was
used for plasmid DNA construction and replication. For most experiments,
cultures of E. coli bearing the indicated plasmids
were grown aerobically in Luria–Bertani (LB)37 at 37 °C supplemented with 100 μg/mL ampicillin
or 50 μg/mL kanamycin, as required, with shaking.33 (link) Bacterial growth was monitored by measuring
the optical density at 600 nm (A600 nm).
coli Stellar (Clontech Laboratories, Mountain View, CA) was
used for plasmid DNA construction and replication. For most experiments,
cultures of E. coli bearing the indicated plasmids
were grown aerobically in Luria–Bertani (LB)37 at 37 °C supplemented with 100 μg/mL ampicillin
or 50 μg/mL kanamycin, as required, with shaking.33 (link) Bacterial growth was monitored by measuring
the optical density at 600 nm (A600 nm).
