Water, acetone, acetonitrile, trifluoroacetic acid (TFA), 2,2′:5′,2″-terthiophene (TER), 1,5-diaminonapthalene (DAN), trans-2-[3-(4-t-butyl-phenyl)-2-methyl-2-propenylidene]malononitrile (DCTB), 4-hydroxy-α-cyano cinnamic acid (CHCA), 4-chloro-α-cyano cinnamic acid (CClCA), Nickel(II) phthalocyanine (NiPc), Nickel(II) 2,9,16,23-tetraphenoxy-29H-31H-phthalocyanine (NiPcPhe), Cu(II) phthalocyanine (CuPc), Zinc(II) Protoporphyrin IX (ZnPP), Cobalt chloride (II) Protoporphyrin IX (CoPP), cytochrome c (horse heart), myoglobin, human hemoglobin, and proteomic-grade trypsin, were obtained from Sigma-Aldrich (Milan, Italy). Livers of cod and bovine as well as mussel were purchased from a local supermarket. Plasma was drawn from a healthy voluntary subject. All solvents used were LC–MS grade. A mass standards kit containing bradykinin (2–9 clip), angiotensin I, glu1-fibrinopeptide, ACTH (1–17, 18–39, 7–38 clips) for calibration was purchased from AB Sciex (Concord, ON, Canada).
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