Single B cell cDNA was generated with random hexamers using SSIII. The antibody heavy- and light-chain variable regions were PCR amplified using AmpliTaq360 master mix (Applied Biosystems). PCR products were purified (Qiagen, Valencia, CA) and sequenced by Genewiz. Gene rearrangements, clonal relatedness, unmutated common ancestors, and intermediate ancestor inferences were made using Cloanalyst (13 (link)). The DH677 clonal lineage tree was generated using FigTree.
Easterhoff D., Pollara J., Luo K., Tolbert W.D., Young B., Mielke D., Jha S., O’Connell R.J., Vasan S., Kim J., Michael N.L., Excler J.L., Robb M.L., Rerks-Ngarm S., Kaewkungwal J., Pitisuttithum P., Nitayaphan S., Sinangil F., Tartaglia J., Phogat S., Kepler T.B., Alam S.M., Wiehe K., Saunders K.O., Montefiori D.C., Tomaras G.D., Moody M.A., Pazgier M., Haynes B.F, & Ferrari G. (2020). Boosting with AIDSVAX B/E Enhances Env Constant Region 1 and 2 Antibody-Dependent Cellular Cytotoxicity Breadth and Potency. Journal of Virology, 94(4), e01120-19.
Antibody B cell Cdna Clonal Gene rearrangementsLight Tree
Corresponding Organization :
Other organizations :
Duke University, Uniformed Services University of the Health Sciences, University of Maryland, Baltimore, Armed Forces Research Institute of Medical Science, Walter Reed Army Institute of Research, Henry M. Jackson Foundation, Ministry of Public Health, Mahidol University, Global Solutions for Infectious Diseases, Sanofi (United States), Boston University
Single B cell cDNA was generated with random hexamers using SSIII.
The antibody heavy- and light-chain variable regions were PCR amplified using AmpliTaq360 master mix (Applied Biosystems).
dependent variables
Gene rearrangements, clonal relatedness, unmutated common ancestors, and intermediate ancestor inferences were made using Cloanalyst (13 (link)).
The DH677 clonal lineage tree was generated using FigTree.
control variables
Control variables not explicitly mentioned.
controls
No positive or negative controls were explicitly mentioned in the input protocol.
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