Kinetics of Virus Infection in Sorted Cells

A cell monolayer was grown in tissue culture dishes (Falcon) for 3 days then infected with MC-RckGFP strain at a MOI of 100:1 for 1 h at 37°C in cell culture medium. After the infection, cells were sorted using flow cytometry. GFP− and GFP+ sorted cells were seeded at a density of 1.5 × 10⁵ cells/ml in culture medium containing inactivated 10% FBS, 10 μg/ml gentamicin and H³-thymidine at 1 μCi/3.76 × 10⁴ Bq (PerkinElmer). The cells were maintained for 90 min, 3 h, 6 h, or 24 h at 37°C in a humidified atmosphere at 5% CO₂ followed by scintillation counting (Packard 1600 TR meter, Meriden, CT) (Olivier et al., 2012 (link)).