Caspase-mediated cell death analysis

Caki-1 cells were treated with mdivi-1 alone, cisplain alone or combined treatments. To measure DNA fragmentation, we used cell death detection ELISA plus kit (Boehringer Mannheim, Indianapolis, IN, USA) according to the manufacturer’s recommendations. For caspase activity assay, cells were harvested and incubated with a reaction buffer containing acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA), acetyl-Ile-Glu-Thr-Asp-p-nitroanilide (Ac-IETD-pNA), or Ac-Leu-Glu-His-Asp-p-nitroaniline (Ac-LEHD-pNA) substrate, as previously described [29 (link)].

Free full text: Click here

Woo S.M., Min K.J, & Kwon T.K. (2020). Inhibition of Drp1 Sensitizes Cancer Cells to Cisplatin-Induced Apoptosis through Transcriptional Inhibition of c-FLIP Expression. Molecules, 25(24), 5793.

Publication 2020

cell death detection ELISA plus kit

Ac devd pna Acetyl val Asp glu Assay Buffer Caspase Cell death Cells Combined treatments Dna fragmentation Elisa Glu asp Nitroaniline

Corresponding Organization : Keimyung University

Other organizations : Daegu-Gyeongbuk Medical Innovation Foundation

Top 5 similar protocols

Variable analysis

independent variables

Mdivi-1 treatment
Cisplatin treatment
Combined treatment of mdivi-1 and cisplatin

dependent variables

DNA fragmentation
Caspase activity (caspase-3, caspase-8, and caspase-9)

control variables

Caki-1 cells

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!