Comprehensive Western Blot Analysis

Western blot assay was performed as previously described.24 (link) The following primary antibodies were used: Tiam1 (Santa Cruz Biotechnology Inc.); vimentin, Snail, Slug, MMP-2, VEGF, and VEGFA (Abcam); E-cadherin and ZO-1 (Cell Signaling Technology); β-Actin (Zhongshan).

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Zhu G., Zhang Y., Wang Q., Che S., Yang Y., Chen L, & Lin Z. (2019). The prognostic value of Tiam1 correlates with its roles in epithelial–mesenchymal transition progression and angiogenesis in lung adenocarcinoma. Cancer Management and Research, 11, 1741-1752.

Publication 2019

Tiam1 vimentin VEGF VEGFA E-cadherin

Actin Antibodies E cadherin Mmp 2 Slug Snail Tiam1 Vegf Vimentin Western blot assay

Corresponding Organization :

Other organizations : Yanbian University of Science and Technology, Yanbian University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Tiam1
Vimentin
Snail
MMP-2
VEGFA
E-cadherin

control variables

None explicitly mentioned

controls

β-Actin (Zhongshan)

Annotations

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