Alginate Extraction from Saccharina latissima

Sodium alginate was extracted from the brown algae Saccharina latissima using a cyclic fractionation process in which diluted sodium citrate was used in 18 sequential extractions from the same biomass [4 (link)]. The alginate fraction used in this work was recovered, by acid precipitation followed by centrifugation, from the 16th extraction cycle. The algae were cultivated on the Swedish west coast and harvested on 17 May 2016, from a cultivation site at the Sven Lovén Centre for Marine Science (University of Gothenburg). The precise extraction protocol is described in a previous work [8 (link)]. Commercial sodium alginate, sodium hydroxide (≥97% ACS reagent, CAS: 9005-38-3), maleic anhydride (MA) (≥99.0% puriss), n-dodecanol (DD) (≥98%, reagent grade), calcium chloride dihydrate (CaCl₂) (≥99% ACS reagent), sodium oxalate (≥99.5% ACS reagent), and phytic acid (≥90% phosphorus basis) were purchased from Sigma-Aldrich, Sweden. Deuterated chloroform (CDCl₃) (99.96%) was purchased from Cambridge Isotope Laboratories, U.S.A. Linseed oil was purchased from Slöjdetaljer, Sweden, and produced by Eskil Åkerberg AB, Staffanstorp, Sweden.