Cellular Trafficking and Lysosomal Modulation

LPS, mevastatin, and FITC-CTB were purchased from MilliporeSigma. Chloroquine, PSC-833, and 2‑hydroxypropyl-βCD were purchased from GE Healthcare (now Cytiva), Valspodar, and InvivoGen, respectively. PRX consisting of 2-(2-hydroxyethoxy) ethyl carbamate–modified βCDs as cyclic molecules, Pluronic P123 as an axle polymer, and N-triphenylmethyl groups as acid-cleavable stopper molecules were synthesized as described previously (45 (link), 46 (link)). The antibodies used were anti-Flag (catalog F1804; MilliporeSigma), anti-GST (PM013; MBL), anti-CFP (D153-3; MBL), anti-Myd88 (sc-74532; Santa Cruz Biotechnology), anti-p65 (sc-372; Santa Cruz Biotechnology), anti-GAPDH (ab181602; Abcam), anti-LAMP1 (sc-20011; Santa Cruz Biotechnology), anti-Rab7 (9367), anti-EE1A (3288; Cell Signaling Technology), anti-PDI (3501; Cell Signaling Technology), anti-Lamin A/C (4777; Cell Signaling Technology), and anti-CTB (SAB4200844; MilliporeSigma).

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Hayakawa S., Tamura A., Nikiforov N., Koike H., Kudo F., Cheng Y., Miyazaki T., Kubekina M., Kirichenko T.V., Orekhov A.N., Yui N., Manabe I, & Oishi Y. (2022). Activated cholesterol metabolism is integral for innate macrophage responses by amplifying Myd88 signaling. JCI Insight, 7(22), e138539.

Publication 2022

LPS anti-Flag anti-Myd88 anti-p65 anti-GAPDH anti-LAMP1 anti-Rab7 anti-PDI anti-Lamin A/C

Acid Antibodies Chloroquine Ethyl carbamate Fitc Gapdh Hydroxypropyl Lamin a c Lamp1 Mevastatin Pluronic p123 Polymer Psc 833 Sc 372 Valspodar

Corresponding Organization : Chiba University

Other organizations : Tokyo Medical and Dental University, Research Institute of General Pathology and Pathophysiology, the Russian Academy of Medical Sciences, National Medical Research Center of Cardiology, Institute of Experimental Cardiology, Institute of Gene Biology, Showa University, Institute for Atherosclerosis Research

Top 5 similar protocols

Variable analysis

independent variables

Mevastatin
FITC-CTB
Chloroquine
PSC-833
2‑hydroxypropyl-βCD
Valspodar

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Anti-Flag
Anti-GST
Anti-CFP
Anti-Myd88
Anti-p65
Anti-GAPDH
Anti-LAMP1
Anti-Rab7
Anti-EE1A
Anti-PDI
Anti-Lamin A/C
Anti-CTB

negative controls

Not explicitly mentioned

Annotations

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