Quantification of 12-MMA in Bl. producta

The content of 12-MMA in the conditioned culture medium of Bl. producta and in the cecal materials and serum of Bl. producta-treated animals was quantified by GC/MS. Preparation of fatty acid methyl esters and GC/MS analysis were performed as previously reported.^{49 (link)} The pure 12-MMA was used as a standard reference. Fatty acid methyl esters were separated using an Elite 5-MS column and monitored by mass spectrometry (Clarus 500-SQ8S, Perkin Elmer, Courtaboeuf, France). The cecal and serum content of acetate was quantified by GC/MS analysis as previously reported.^{50 (link)} N-methylbenzylamine-d0/d3 was applied as the chemical derivatization reagent to enhance the sensitivity and accuracy.

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Xu W., Yu J., Yang Y., Li Z., Zhang Y., Zhang F., Wang Q., Xie Y., Zhao B, & Wu C. (2023). Strain-level screening of human gut microbes identifies Blautia producta as a new anti-hyperlipidemic probiotic. Gut Microbes, 15(1), 2228045.

Publication 2023

Clarus 500-SQ8S

Acetate Animals Cecal Conditioned culture medium Esters Fatty acid Gc ms Mass spectrometry N methylbenzylamine Sensitivity Serum

Corresponding Organization : Tianjin University of Traditional Chinese Medicine

Other organizations : Chinese Academy of Medical Sciences & Peking Union Medical College

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Variable analysis

independent variables

Bl. producta

dependent variables

Content of 12-MMA in the conditioned culture medium of Bl. producta
Content of 12-MMA in the cecal materials of Bl. producta-treated animals
Content of 12-MMA in the serum of Bl. producta-treated animals
Cecal content of acetate
Serum content of acetate

control variables

Use of pure 12-MMA as a standard reference
Fatty acid methyl esters separation using an Elite 5-MS column
Mass spectrometry monitoring (Clarus 500-SQ8S, Perkin Elmer, Courtaboeuf, France)
N-methylbenzylamine-d0/d3 used as the chemical derivatization reagent

controls

Pure 12-MMA used as a standard reference

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