Tissue Microarray-based IGF-IR Evaluation

Tissue microarrays (TMAs) with duplicate 1 mm cores were constructed from representative tumor regions of formalin-fixed paraffin-embedded tissue blocks, as previously described (27 (link)). Immunohistochemical staining was performed on 4 µm deparaffinized and pre-treated TMA sections using an automatic immunostainer (TechMate™500 Plus, DAKO, Glostrup, Denmark), DAKO Envision, and anti-IGF-IRβ antibody (1:150, Santa Cruz Biotechnology, Cat. No.: sc-713). The IGF-IR membrane (IGF-IR^m) expression was evaluated by two independent observers (AR, SBj) and scored as either negative or any positive membrane staining.

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Rosendahl A.H., Björner S., Ygland Rödström M., Jirström K., Borgquist S., Ingvar C., Pollak M.N, & Jernström H. (2021). Pre- and Postoperative Circulating IGF-I, IGFBP-3, and IGFBP-7 Levels in Relation to Endocrine Treatment and Breast Cancer Recurrence: A Nested Case-Control Study. Frontiers in Oncology, 11, 626058.

Publication 2021

TechMate™500 Plus Envision anti-IGF-IRβ antibody

Anti antibody Embedded paraffin Formalin Igf ir Membrane Microarrays Regions blocks Tissue Tumor

Corresponding Organization :

Other organizations : Lund University, Skåne University Hospital, Aarhus University Hospital, Jewish General Hospital, McGill University

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Variable analysis

independent variables

Tissue microarray (TMA) construction with duplicate 1 mm cores from representative tumor regions of formalin-fixed paraffin-embedded tissue blocks

dependent variables

IGF-IR membrane (IGF-IR^m) expression, evaluated by two independent observers and scored as either negative or any positive membrane staining

control variables

Immunohistochemical staining performed on 4 µm deparaffinized and pre-treated TMA sections using an automatic immunostainer (TechMate™500 Plus, DAKO, Glostrup, Denmark), DAKO Envision, and anti-IGF-IRβ antibody (1:150, Santa Cruz Biotechnology, Cat. No.: sc-713)

controls

No specific positive or negative controls were mentioned in the input.

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