Comparative Analysis of Gene Expression in NAFLD

The gene expression datasets re-analyzed in this study were obtained from the GEO database (https://www.ncbi.nlm.nih.gov/geo/). We selected two gene expression profiles (GSE63067, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE63067, PMID: 25993042 and GSE89632, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE89632, PMID: 25581263) related to Homo sapiens’ NAFLD from the database. Among them, GSE63067 was based on the Agilent GPL570 platform ([HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array), and GSE89632 was based on platform GPL14951 (Illumina HumanHT-12 WG-DASL V4.0 R2 expression beadchip). GSE63067 (13 (link)) included 18 participants (2 SS, 9 NASH, 7 HC). Liver samples were obtained during laparoscopic bariatric surgery (for NAFLD) or laparoscopic cholecystectomy (for HC). GSE89632 (12 (link)) included 63 participants (20 SS, 19 NASH, 24 HC), and liver tissues were collected during percutaneous needle biopsy (for NAFLD) or as a wedge biopsy during hepatectomy (for HC). All of the data were freely available online.

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Chen L., Chen L., Li X., Qin L., Zhu Y., Zhang Q., Tan D., He Y, & Wang Y.H. (2023). Transcriptomic profiling of hepatic tissues for drug metabolism genes in nonalcoholic fatty liver disease: A study of human and animals. Frontiers in Endocrinology, 13, 1034494.

Publication 2022

Human Genome U133 Plus 2.0 Array HumanHT-12 WG-DASL V4.0 R2 expression beadchip

Bariatric surgery Biopsy Gene expression Hepatectomy Homo sapiens Human genome Laparoscopic Laparoscopic cholecystectomy Liver Nafld Nash Needle biopsy Tissues

Corresponding Organization : Zunyi Medical University

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Variable analysis

independent variables

NAFLD status (SS, NASH, HC)

dependent variables

Gene expression profiles

control variables

Liver sample collection method (laparoscopic bariatric surgery or laparoscopic cholecystectomy, and percutaneous needle biopsy or wedge biopsy during hepatectomy)
Sequencing platform (Agilent GPL570 and Illumina HumanHT-12 WG-DASL V4.0 R2)

controls

Positive control: Liver samples from participants with NAFLD (SS and NASH)
Negative control: Liver samples from healthy controls (HC)

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