Transcriptome Profiling of Wheat Cultivars

Using RNA samples with Integrity Number (RIN) ≥ 7, cDNA libraries of YD588, YN21, and the resistant and susceptible bulks were constructed. RNA integrity assessment and cDNA libraries construction were performed based on the previous studies (Zhu et al., 2020 (link)). Then, using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, United States), the quality of the cDNA libraries was assessed. The cDNA libraries sequencing was carried out on the Illumina HiSeq sequencing platform (Illumina HiSeq4000) by Beijing Biomics Technology Company Limited (Beijing, China). High-quality clean data were obtained after raw data filtering, sequences joint, and poor-quality reads elimination. The 10 and 20 Gb clean data were set for parents and bulks, respectively, in the sequencing indicator. Following mapping, the clean data to the reference genome of Chinese Spring (v1.1),¹ SNP calling, DEG analysis, and gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed in Cloud Platform developed by Beijing Biomics Technology Company Limited.