Drosophila Genetic Reagents Utilized

Previously described fly strains used in this work are 1: w¹¹¹⁸, 2: dhipk-Gal4 (hipk[BG00855], BDSC #12779), 3: UAS-GFP (BDSC #5431), 4: UAS-pc^RNAi (BDSC #33964), 5: UAS-e(z)^RNAi (BDSC #36068), 6: UAS-sce^RNAi (BDSC #67924), 7: UAS-ph-d^RNAi (BDSC #63018) 8: dhipk⁴ (Lee et al. 2009a (link)), 9: dpp-Gal4/TM6B (Staehling-Hampton et al. 1994 (link)), 10: UAS-HA-dhipk^attp40 (Tettweiler et al. 2019 (link)), 11: eyFLP; act>y⁺>Gal4, UAS-GFP (Pagliarini and Xu 2003 (link)). The details of how UAS-myc-hHIPK1^attp40, UAS-myc-hHIPK2^attp40, UAS-myc-hHIPK3^attp40, and UAS-myc-hHIPK4^attp40 were generated for this work is detailed in the section titled “Generation of plasmids and transgenic UAS-hHIPK fly stocks.” dhipk mutant rescue experiments were performed at 18°C and 25°C to determine the ideal Hipk expression levels by modulating the expression of Gal4-driven UAS-Hipk constructs, while experiments using dpp-Gal4 were performed at 29°C to increase UAS-Hipk expression. Flies were raised on standard media composed of 0.8 g agar, 2.3 g yeast, 5.7 g cornmeal, and 5.2 mL molasses per 100 ml. “BDSC” is an acronym for the Bloomington Drosophila Stock Center.