Protein Expression Profiling in Cell Lines

The procedures for protein extraction, protein concentration determination, conventional SDS-PAGE, protein band visualization, and protein band intensity measurement were performed according to our reported methods [20 ,21 (link)]. The used antibodies which were recognized included Bcl-2 (1:1000), Mcl-1 (1:1000), Noxa (1:1000), Bax (1:1000), PERK (1:1000), eIF2α (1:1000), LC3 (1:1000), p62 (1:1000) (Santa Cruz Biotechnology, Santa Cruz, CA, USA), P-PERK (1:500, Threonine-982), P-YAP1 (1:500, Serine-127) (Abcam, Cambridge, UK), P-eIF2α (1:500, Serine-51), YAP1 (1:1000) (Cell Signaling, Danvers, MA, USA), and Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH, 1:3000) (R&D Systems, Minneapolis, MN, USA).