Identification of Insect Neuropeptide Sequences

The cDNA and translated protein of each neuropeptide were selected by searching for the sequences of closely related orthopteran species against the in-house database of G. bimaculatus transcripts constructed in previous reports [12 (link)]. Among the 43 neuropeptides that were reported in insects, 35 neuropeptides were provisionally determined as the reference neuropeptide sequences, which are the mature, precursor, or cDNA sequences (Table S1). In particular, the sequences of ACP [7 (link)], Ast B [12 (link)], corazonin [6 (link)], elevenin [6 (link)], myosuppressin [13 (link)], and PDF [14 (link)] were used as seed sequences as described in previously published studies. These selected proteins, except for sequences published in previous studies, were submitted with BLASTP [24 (link)] on the NCBI website (https://blast.ncbi.nlm.nih.gov/Blast.cgi (accessed on 16 July 2022)) with the clustered nr database to confirm homology with the sequences of closely related orthopteran species. These precursor proteins and their amino acid sequences deduced from cDNA were confirmed to belong to that they belong to appropriate protein clusters (Table S2).

Free full text: Click here

Mochizuki T., Sakamoto M., Tanizawa Y., Seike H., Zhu Z., Zhou Y.J., Fukumura K., Nagata S, & Nakamura Y. (2023). Best Practices for Comprehensive Annotation of Neuropeptides of Gryllus bimaculatus. Insects, 14(2), 121.

Publication 2023

Amino acid sequences Cdna Corazonin Homology sequences Insects Neuropeptide Precursor proteins Protein

Corresponding Organization : Research Organization of Information and Systems

Other organizations : The University of Tokyo

Top 5 similar protocols

Variable analysis

independent variables

Sequences of closely related orthopteran species
Mature, precursor, or cDNA sequences of 35 neuropeptides

dependent variables

Homology of the selected proteins with the sequences of closely related orthopteran species

control variables

In-house database of G. bimaculatus transcripts constructed in previous reports [12]
Seed sequences of ACP [7], Ast B [12], corazonin [6], elevenin [6], myosuppressin [13], and PDF [14]

positive controls

Seed sequences of ACP [7], Ast B [12], corazonin [6], elevenin [6], myosuppressin [13], and PDF [14]

negative controls

Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!