Multiparametric Flow Cytometry Assay

All flow cytometry was performed using LSRFortessa Flow Cytometer (BD Biosciences). GFP linked shRNA competition assays were performed as described previously(80 (link)). Apoptosis was assayed using APC-Annexin V (Tonbo biosciences) or Annexin V-FITC Kit (Miltenyi Biotec). All data were processed and analyzed with FCS Express 7 Research Edition (De Novo Software, Inc; RRID:SCR_016431). All data plotted with GraphPad (Prism, RRID:SCR_002798).

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Patel A.J., Warda S., Maag J.L., Misra R., Miranda-Román M.A., Pachai M.R., Lee C.J., Li D., Wang N., Bayshtok G., Fishinevich E., Meng Y., Wong E.W., Yan J., Giff E., Pappalardi M.B., McCabe M.T., Fletcher J.A., Rudin C.M., Chandarlapaty S., Scandura J.M., Koche R.P., Glass J.L., Antonescu C.R., Zheng D., Chen Y, & Chi P. (2022). PRC2-Inactivating Mutations in Cancer Enhance Cytotoxic Response to DNMT1-Targeted Therapy via Enhanced Viral Mimicry. Cancer Discovery, 12(9), 2120-2139.

Publication 2022

LSRFortessa Flow Cytometer APC-Annexin V Annexin V-FITC Kit FCS Express 7 Research Edition

Annexin v Annexin v fitc Apoptosis Flow cytometry Prism Shrna

Corresponding Organization : Cornell University

Other organizations : Albert Einstein College of Medicine, GlaxoSmithKline (United States), Brigham and Women's Hospital, Harvard University

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Variable analysis

independent variables

ShRNA (GFP linked)

dependent variables

Apoptosis (measured using APC-Annexin V or Annexin V-FITC)

control variables

Flow cytometry performed using LSRFortessa Flow Cytometer (BD Biosciences)
Data processed and analyzed with FCS Express 7 Research Edition (De Novo Software, Inc)

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