Characterization of Drug Metabolism Enzymes

Osimertinib and AZ5104 were purchased from Beijing Sunflower and Technology Development Co., Ltd. (Beijing, China). Omeprazole, lansoprazole, and rabeprazole were purchased from Shanghai Canspec Scientific Instruments Co., Ltd. (Shanghai, China). Sorafenib was purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Pooled RLMs and HLMs were from Corning Life Sciences Co., Ltd. Recombinant human CYP3A4 and cytochrome b5 were prepared by our group as indicated previously (Zhou et al., 2019 (link)). Reduced nicotinamide adenine dinucleotide phosphate (NADPH) was purchased from Roche Pharmaceutical Ltd. (Basel, Switzerland). All other chemicals and solvents not mentioned were of analytical grade. The information on 114 drugs is presented in Supplementary Table S1.

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Gao N., Zhang X., Hu X., Kong Q., Cai J., Hu G, & Qian J. (2022). The Influence of CYP3A4 Genetic Polymorphism and Proton Pump Inhibitors on Osimertinib Metabolism. Frontiers in Pharmacology, 13, 794931.

Publication 2022

Sorafenib Reduced nicotinamide adenine dinucleotide phosphate (NADPH)

Az5104 Cyp3a4 Cytochrome b5 Drugs Human Lansoprazole Nicotinamide adenine dinucleotide phosphate Omeprazole Osimertinib Rabeprazole Solvents Sorafenib Sunflower

Corresponding Organization : Wenzhou Medical University

Other organizations : Wenzhou City People's Hospital

Top 5 similar protocols

Variable analysis

independent variables

Osimertinib
AZ5104
Omeprazole
Lansoprazole
Rabeprazole
Sorafenib

dependent variables

Not explicitly mentioned

control variables

Pooled RLMs and HLMs
Recombinant human CYP3A4 and cytochrome b5
Reduced nicotinamide adenine dinucleotide phosphate (NADPH)

controls

Positive control: Recombinant human CYP3A4 and cytochrome b5
Negative control: Not specified

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