DR0401 protein was purified from insect cell cultures as previously described58 (link),59 (link). Monomers were loaded with 0.2 mg/ml peptide at 37 °C for 72 h in the presence of 0.2 mg/ml n-Dodecyl-β-maltoside and 1 mM Pefabloc (Sigma–Aldrich). Peptide-loaded monomers were conjugated into tetramers using R-PE streptavidin (Invitrogen), PE-Cy5 streptavidin (BD), or PE-CF594 streptavidin at a molar ratio of 8:1.
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