In all, 6–8-weeks-old C57BL/6 mice were used to induce peritonitis by intraperitoneal administration of 1 mg MSU crystals (dissolved in 0.5 ml PBS). Before injection of MSU, 20 mg/kg Ori (dissolved in vehicle containing 90% PBS and 10% DMSO) were injected intraperitoneally. After 6 h, the mice were killed and 10 ml ice-cold PBS were used to wash the peritoneal cavities. The polymorphonuclear neutrophils in peritoneal lavage fluid was analyzed by flow cytometry by staining Ly6G and CD11b. The IL-1β level in serum or peritoneal lavage fluid was determined by ELISA.
For inducing joint inflammation, mice were administered Ori (20 mg/kg, dissolved in DMSO) by intraarticular injection. After 30 min, 0.5 mg MSU (dissolved in 20 μL PBS) was administrated intraarticularly and then the size of joints was measured at different time points. After 24 h, the patella were isolated and cultured in 200 μl opti-MEM medium containing 1% Penicillin-Streptomycin at room temperature for 1 h.
For inducing joint inflammation, mice were administered Ori (20 mg/kg, dissolved in DMSO) by intraarticular injection. After 30 min, 0.5 mg MSU (dissolved in 20 μL PBS) was administrated intraarticularly and then the size of joints was measured at different time points. After 24 h, the patella were isolated and cultured in 200 μl opti-MEM medium containing 1% Penicillin-Streptomycin at room temperature for 1 h.
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