Cultivation of Ovarian Cancer Cell Lines

Human ovarian cancer cell lines HEY, HEY A8, A2780, SKOV3 and SKOV3 ip1[43 (link)] were purchased from ATCC. A2780/CDDP and an immortalized normal human ovarian epithelial cell line (T29) were maintained in the laboratory of cancer Institute, Fudan University Shanghai Cancer Center. HEY A8, A2780/CDDP and SKOV3 ip1 derived from HEY, A2780 and SKOV3, respectively, are resistant to cisplatin or paclitaxel [44 (link), 45 (link)]. Cells were routinely cultured with RPMI 1640 supplemented with 10% fetal bovine serum (FBS), antibiotics (100U/mL penicillin and 100μg/mL streptomycin) in a humidified incubator at 37°C and 5% CO₂. Both RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Thermo Scientific.