At 12.5 dpc, dams were administered an i.p. injection of poly (I:C) (Sigma Aldrich, St. Louis, MO) at 4 mg/kg or 20 mg/kg based on the weight of the poly (I:C) itself [8 (link)]. A model of neurodevelopmental disorder using MIA resulted in severe MIA equivalent to fulminant infection in human [8 (link), 10 (link), 18 (link)–20 (link)]. In clinical settings, all the infected mothers would not suffer from fulminant infection. Therefore, we also examined the effects of comparatively low dose of poly (I:C). In this study, the groups injected with poly (I:C) at 4 mg/kg or 20 mg/kg are presented as Poly 4 and Poly 20, respectively. We injected intraperitoneally mouse recombinant LIF (rLIF; Millipore, Billerica, MA) into dams at 5 μg/kg body weight [14 (link)] at 12.5 or 13.5 dpc. The injection volume was unified into 0.01 ml/g. Control was injected with 0.01 ml/g volume of only saline.
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