Pyroptosis-Related Genes Analysis in Ovarian Cancer

We extracted 33 pyroptosis-related genes from prior reviews^{17 (link)–20 (link)}, and they are presented in Table S1. Due to the lack of normal ovarian tissue data in the TCGA cohort, we also considered GTEx data from 88 normal ovarian samples to identify the DEGs between normal and tumour tissues. The expression data in both datasets were normalized to fragment per kilobase million (FPKM) values before comparison. The “limma” package was used to identify DEGs with a P value <0.05. The DEGs are notated as follows: * if P < 0.05, ** if P < 0.01, and *** if P < 0.001. A PPI network for the DEGs was constructed with Search Tool for the Retrieval of Interacting Genes (STRING), version 11.0 (https://string-db.org/).