The activity of GPx and the calculation of its determination were carried out as described by [21 (link)] using a glutathione reductase activity kit (Enzo Life Sciences®, Butler Pike Plymouth Meeting, PA, USA). In brief, 50 μL of hippocampal supernatant diluted 1:5, blank or GR standard; 100 μL of Master Mix (10X GR buffer, GSSG reagent, and distilled water); and 100 μL of NADP solution were added to each well to initiate the reaction. The absorbance was measured at 340 nm every minute for 10 min at room temperature. The data were expressed in U/mg of protein [21 (link)].
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