Oral Glucose Tolerance Test in Mice

Oral glucose tolerance test was performed according to a previously described method with some modifications (Horakova et al., 2016 (link)). The oral glucose tolerance test is considered to be a method for analyzing the homeostasis of circulating glucose in mice. In brief, at the end of the second week, the mice were fasted for 6 h (7:00 am1:00 pm) and blood samples were collected. Insulin levels were measured by commercial ELISA analysis kit according to the manufacturer’s instructions. After oral administration of glucose, the blood glucose meter (Sinocare Inc., Changsha, China) was immediately used to measure the glucose level from the tail blood before 0 (in rapid state) or after 15, 30, 60, and 120 min.