Western Blot Analysis of HCV Proteins

Western blot (WB) was performed as previously described [25 (link)]. Briefly, after the membrane was washed, the target proteins were accordingly probed with the antibody to HCV Core (1 : 2500) or NS3 (1 : 2500). As an internal control, the antibody to actin (1 : 4000) was used. After having been washed with TBST, the membrane was incubated with the goat anti-mouse or goat anti-rabbit secondary antibody, respectively. The proteins were detected using an Immobilon Western Chemiluminescent HRP Substrate (Millipore, Inc.) with ChemiDo XRS gel imager system (Bio-Rad, CA). The protein signal intensity was scanned with the Gelpro32 software, and a ratio of the interested protein to the internal control protein actin was calculated and normalized as 1.00 for the control group.