Culturing Cell Lines and Bacterial Strains

LX-2, HT-29, and THP-1 cells were obtained from the American Type Culture Collection (ATCC; Manassas, VA). Cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco), 100 U/mL penicillin, and 100 mg/mL streptomycin. C29 was purchased from Med Chem Express (NJ, USA). In this study, the most common clinical strain of Enterococcus was used (40 (link), 41 (link)). Enterococcus faecalis was grown in LB agar powder (Sango Biotech) in a shaker at 37°C for 8 h.

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Zhang X., Liang Y., Jiang J., Lu C., Shi F., Cao Q., Zhang Y, & Diao H. (2023). A High-Salt Diet Exacerbates Liver Fibrosis through Enterococcus-Dependent Macrophage Activation. Microbiology Spectrum, 11(2), e03403-22.

Publication 2023

THP-1 RPMI 1640 medium FBS LB agar powder

Agar Cells Cultured medium Enterococcus Enterococcus faecalis Fetal bovine serum Ht 29 Penicillin Powder Strain Streptomycin Thp 1 cells

Corresponding Organization : First Affiliated Hospital Zhejiang University

Other organizations : Hangzhou Normal University, Wenzhou Medical University, First Affiliated Hospital of Wenzhou Medical University, Zhejiang University

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Variable analysis

independent variables

Cell lines (LX-2, HT-29, and THP-1)
Compound (C29)

dependent variables

Unspecified

control variables

Cell culture medium (RPMI 1640 supplemented with 10% FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin)
Bacterial strain (Enterococcus faecalis)

controls

Positive control: The most common clinical strain of Enterococcus (references provided)
Negative control: Not specified

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