Cytofluorometric Analysis of HCMV-Infected OECs
Corresponding Organization :
Other organizations : Université de franche-comté, Centre Hospitalier Universitaire de Besançon
Variable analysis
- Infection status: uninfected OECs, HCMV-infected OECs, and CTO cells
- Cell cycle analysis: measured using propidium iodide staining and flow cytometry
- Cell number: 1 × 10^5 cells collected for each condition
- Staining protocol: cells were fixed, permeabilized, and stained as previously reported [19]
- Flow cytometry: BD LSRFortessa X-20 flow cytometer used for analysis, FACSDiva software used for data collection and analysis
- Positive control: not explicitly mentioned
- Negative control: not explicitly mentioned
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