Cytofluorometric Analysis of HCMV-Infected OECs

Cells (1 × 10⁵) were collected from uninfected OECs, HCMV-infected OECs and CTO cells. Cells were fixed, permeabilized, and stained as previously reported [19 (link)]. Cytofluorometric analysis was achieved using a BD LSRFortessa X-20 (BD Biosciences) flow cytometer. FACSDiva software (BD Biosciences) was used for data collection and analysis. The antibodies used are provided in Supplementary Table 2. For cell cycle analysis, uninfected OECs or CTO cells were washed in 1X PBS, fixed in 70% ethanol, and resuspended in 50 µg/ml propidium iodide (P3566, life technologies, Eugene, USA) with 0.1 mg/ml RNase (R4642, Sigma-Aldrich, Saint-Louis, MO, USA), then incubated at 37 °C for 30 min as described previously [17 (link)].