We used male SD (Sprague Dawley) rats (License No. SCXK 2016-0011) weighing 200–220 g supplied by the Beijing Vital River Laboratory Animal Technology Co., Ltd. Rats were reared at 25 ± 1°C and 65 ± 5% temperature and humidity, with a 12 h light-dark cycle. All rats were adapted to the environment for about one week and had free access to food and water. Try to minimize animal suffering during experiments. The Committee approved the procedures and ethics guidelines for Experimental Animal Use and Care of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China.
Rats were randomly assigned to five groups (n = 10): control group, model group, fluoxetine (10 mg kg−1) group (Liang et al., 2015 (link); Sun et al., 2017 (link)), JDTL low group (2 g kg−1) and JDTL high group (4 g kg−1). The JDTL Granules and fluoxetine were intragastrically administrated from the first day of the surgery until the behavioral test. Rats in the control and the model group were given the same volume of drinking water. The dosage of drugs was updated according to the weight of rats weekly. Fluoxetine hydrochloride obtained from Lilly (NO. 5198A, Suzhou, China).
Rats were randomly assigned to five groups (n = 10): control group, model group, fluoxetine (10 mg kg−1) group (Liang et al., 2015 (link); Sun et al., 2017 (link)), JDTL low group (2 g kg−1) and JDTL high group (4 g kg−1). The JDTL Granules and fluoxetine were intragastrically administrated from the first day of the surgery until the behavioral test. Rats in the control and the model group were given the same volume of drinking water. The dosage of drugs was updated according to the weight of rats weekly. Fluoxetine hydrochloride obtained from Lilly (NO. 5198A, Suzhou, China).
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