Thyroid Cancer Cell Lines Characterization

The human thyroid cells employed consisted of papillary carcinoma (TPC-1, KTC-1; radiation-sensitive), follicular carcinoma (WRO) and anaplastic carcinoma (FRO and KTC-2; radiation-resistant) cells [9 (link)]. Table 1 shows the characteristics of the cell lines. Cells were cultured in Dulbecco’s modified Eagle’s medium (high-glucose) and nutrient mixture F-12 HAM (Fujifilm Wako Pure Chemical, Doshomachi, Osaka, Japan) (1:1) supplemented with 5% fetal bovine serum (FBS; Equitech-Bio, Inc. Kerrville, TX, USA) in humidified 5% CO₂ at 37°C.

Free full text: Click here

Ihara M., Shichijo K., Ashizawa K., Matsuda K., Otsubo R., Horie I., Nakashima M, & Kudo T. (2020). Relationship between thyroid tumor radiosensitivity and nuclear localization of DNA-dependent protein kinase catalytic subunit. Journal of Radiation Research, 61(4), 511-516.

Publication 2020

F-12 HAM

Anaplastic carcinoma Cell lines Cells Cultured medium Eagle Follicular carcinoma Glucose Human Nutrient Papillary carcinoma Radiation Thyroid

Corresponding Organization : Nagasaki University

Top 5 similar protocols

Variable analysis

independent variables

Cell lines (papillary carcinoma TPC-1, KTC-1; follicular carcinoma WRO; anaplastic carcinoma FRO, KTC-2)

dependent variables

Not explicitly mentioned

control variables

Culture medium (Dulbecco's modified Eagle's medium (high-glucose) and nutrient mixture F-12 HAM (1:1))
Fetal bovine serum (5%)
Incubation conditions (humidified 5% CO2 at 37°C)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!