Flow Cytometry Microglia Immunophenotyping
Corresponding Organization :
Other organizations : Harbin Medical University
Variable analysis
- Anti-murine CD16/CD32 Fc-Receptor blocking reagent
- FITC-conjugated mouse anti-rat CD11b antibody
- PerCP/Cy5.5-conjugated anti-rat CD45 antibody
- Anti-iNOS antibody
- Rabbit mAb anti-arginase-1 (Arg-1) antibody
- PE-conjugated anti-rabbit IgG (H+L) secondary antibody
- INOS expression
- Arginase-1 (Arg-1) expression
- CD11b+/CD45low microglia expression
- Cell suspension incubation time with anti-murine CD16/CD32 Fc-Receptor blocking reagent (10 min)
- Microglia fixation and permeabilization time (20 min)
- Microglia incubation time with primary and secondary antibodies (30 min)
- Number of events recorded (10,000)
- None specified
- None specified
Annotations
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