Immunostaining of Tissue Sections

Immunostaining of tissue sections was performed as previously described [17 (link)]. IF was conducted using the antibodies p73 EP436Y (Abcam ab40658; 1:1000; Cambridge, UK) and p63α D2K8X (Cell Signaling Technology #13109; 1:1000; Danvers, MA), with TSA Plus Fluorescence Amplification Kit (Perkin Elmer, Waltham MA). Nuclei were counterstained with Slowfade Gold DAPI mounting media (Invitrogen, Carlsbad, CA). Micrographs of human tissues were all taken using a Zeiss slide scanner and further analyzed using Zeiss software. Micrographs of murine tissues were taken using a Leica microscope and camera and analyzed using Leica software; all scale bars = 50 µm.

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Marshall C.B., Beeler J.S., Lehmann B.D., Gonzalez-Ericsson P., Sanchez V., Sanders M.E., Boyd K.L, & Pietenpol J.A. (2021). Tissue-specific expression of p73 and p63 isoforms in human tissues. Cell Death & Disease, 12(8), 745.

Publication 2021

ab40658 p63α D2K8X TSA Plus Fluorescence Amplification Kit Leica microscope and camera

Antibodies Dapi Fluorescence Gold Human Microscope Murine Nuclei Tissues

Corresponding Organization :

Other organizations : Vanderbilt University, Vanderbilt University Medical Center, Gilead Sciences (United States)

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Variable analysis

independent variables

P73 EP436Y (Abcam ab40658; 1:1000; Cambridge, UK) antibody
P63α D2K8X (Cell Signaling Technology #13109; 1:1000; Danvers, MA) antibody

dependent variables

Immunostaining of tissue sections

control variables

Tissue sections (human and murine)
Slowfade Gold DAPI mounting media (Invitrogen, Carlsbad, CA)
Zeiss slide scanner and software (for human tissues)
Leica microscope, camera, and software (for murine tissues)

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