C. glabrata Infection Protocol in Mice

The fungal tissue burden and expression analysis were performed as described previously.^{16 (link)} Five male BALB/c mice aged 7 weeks (Japan SLC Inc.) were used for a group. To establish C. glabrata infection, mice were injected into their tail vein with saline suspensions of 1 × 10⁷ viable cells (in a volume of 200 μL). After 7 days, mice were sacrificed by CO₂ inhalation, and target organs (kidney and spleen) were excised aseptically, individually weighed and homogenized in sterile saline using a 70 μm Cell strainer (BD Falcon, Franklin Lakes, NJ, USA). Organ homogenates were diluted and plated onto YPD containing streptomycin sulphate salt (Sigma–Aldrich), penicillin G sodium salt (Sigma–Aldrich), 20 mg/L cholesterol and 0.5% Tween 80-ethanol. Colonies were counted after 1 day of incubation at 37°C and the numbers of cfu/g of the organ were calculated. Statistical analyses were conducted using GraphPad Prism6^™ via an unpaired t-test with a significance level of P < 0.05.