Comprehensive RNA Isolation from Various Biofluids

The samples used were from a previously published study (Yeri et al., 2017 (link)). Plasma and saliva samples were isolated (1 ml) using the mirVana miRNA Isolation Kit (ThermoFisher Scientific, Waltham, MA, AM1560) according to (Burgos et al., 2013 (link)). Samples were DNase treated using TURBO DNA-free Kit (ThermoFisher Scientific, Waltham, MA, AM 1907). Samples were then cleaned and concentrated using Zymo RNA Clean and Concentrator (Zymo Research, Irvine, CA, R1016) using Protocol: Purification of small and large RNAs into separate fractions and combining the fractions at the end. Urine samples (15 ml) were isolated with Urine Total RNA Purification Maxi Kit, Slurry Format (Norgen Biotek Corp., Thorold, ON, Canada, Cat#29600). Samples were DNase treated on column using RNase-Free DNase Set (Qiagen, Germantown, MD, cat# 79254). Samples were concentrated by Speed Vacuum.