All procedures were performed in accordance with the guidelines of the National Institutes of Health animal care committee. One hundred and one thousand HepG2 cells cultured for one week in the presence or absence of varying concentrations of Dox were re-suspended in 200 μl DMEM and Matrigel (BD Bioscience) (1:1) and injected into flanks of NOD/SCID mice (Frederick National Laboratory for Cancer Research). Dox in concentrations of 10 ng/ml and 100 ng/ml was added to drinking water containing 1% sucrose to reduce a bitter taste every two days. Tumor formation was monitored weekly by palpation for 8 weeks. Limiting dilution analysis was performed as described (3 (link)) (http://bioinf.wehi.edu.au/software/elda/index.html ).
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Inducible Tumor Formation in Mice
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Corresponding Organization :
Other organizations : Center for Cancer Research, National Cancer Institute, Johannes Gutenberg University Mainz, University of Copenhagen
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Variable analysis
independent variables
- Dox concentration (10 ng/ml and 100 ng/ml)
- Tumor formation
- Drinking water containing 1% sucrose
- Injection of HepG2 cells into flanks of NOD/SCID mice
- Culture duration of one week for HepG2 cells
- Positive control: HepG2 cells cultured in the presence of Dox
- Negative control: HepG2 cells cultured in the absence of Dox
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