Live-cell imaging was performed using the same protocol and sample preparation as described by Prusicki et al. (2019) (link). Up to 10 samples including WT control next to the mutants were followed in the same Petri dish. A W-plan-Apochromat 40×/1.0 differential interference contrast water-immersion objective on a Zeiss LSM880 confocal microscope with Zen 2.3 SP1 software (Carl Zeiss) permitted the time-lapse acquisition. mTurquoise2 was excited at λ 458 nm and detected at λ 460–510 nm; GFP was excited at 488 nm and detected at 495–560 nm; mVenus was excited at 514 nm and detected 520–620 nm; and TagRFP was excited at 561 nm and detected at 570–650 nm. Time lapses were acquired as series of eight Z-stacks with 4-µm intervals (step size) using fluorescence autofocusing. Acquisitions were performed at 18°C. Image drift on Z plane was corrected manually using the review multidimensional data option on Metamorph v7.8. Image drift on XY plane was corrected using the Stack Reg plugin (Rigid Body option) of Fiji.