The apoptotic effects
of compound 2 and compound 4 on HL-60 cells were investigated using
the annexin V detection kit (BioLegend) in accordance with the manufacturer’s
instructions. The human promyelocytic leukemia cell line was plated
in 6-well plates at a density of 1.2 × 105 cells/well.
The cells were exposed to the active substances (2 and 4 ) at an IC50 concentration for 24 h at 37 °C
and 5% CO2. Next, the cells were centrifuged at 200 rmp
and washed twice with PBS buffer. The pellets were resuspended and
stained with propidium iodide and annexin V in annexin binding buffer
(ABB). After double staining, the cells were incubated in the dark
for 30 min and analyzed in a flow cytometry analyzer (BD FACSCelesta).15 (link)
of compound 2 and compound 4 on HL-60 cells were investigated using
the annexin V detection kit (BioLegend) in accordance with the manufacturer’s
instructions. The human promyelocytic leukemia cell line was plated
in 6-well plates at a density of 1.2 × 105 cells/well.
The cells were exposed to the active substances (
and 5% CO2. Next, the cells were centrifuged at 200 rmp
and washed twice with PBS buffer. The pellets were resuspended and
stained with propidium iodide and annexin V in annexin binding buffer
(ABB). After double staining, the cells were incubated in the dark
for 30 min and analyzed in a flow cytometry analyzer (BD FACSCelesta).15 (link)
