Purified peptides were dissolved at a final concentration of 1.3 mM in acidic conditions in 40% acetonitrile and 0.1% TFA (room temperature). Peptides were incubated for desired assembly times.
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Protocol detail
Peptide Dissolution and Assembly
Corresponding Organization :
Other organizations : Applied Physical Sciences (United States), Emory University
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Variable analysis
independent variables
- Assembly time
- Peptide concentration
- Peptide concentration (1.3 mM)
- Solvent (40% acetonitrile and 0.1% TFA)
- Temperature (room temperature)
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