Trophozoites were incubated for 24 h at 37 °C with stevioside at a concentration of 9.53 mM leaving trophozoites under normal culture conditions. After the incubation, trophozoites were harvested by cooling at 4 °C for 15 min. Trophozoites (2.5 × 105) were seeded in 24-well plates treated with 100 μg fibronectin. Adhesion assays were carried out at 15, 30, and 60 min. Cell viability was evaluated by trypan blue, and the percentage of adhered cells was determined by a light microscope.
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