Heat Shock Response in Nematodes

During the heat shock assay, the nematodes of L4 period were firstly divided into magnolol group and control group, with the number of nematodes in each group around 50. Prior to exposure to the heat exciters, the nematodes in the thymol group were placed on Petri dishes containing magnolol for 5 days of incubation, while the blank control nematodes were placed on control plates containing DMSO for 5 days of incubation. After 5 d of drug treatment, the nematodes were placed on new NGM and incubated at 35 °C. The number of nematode death was recorded every two hours until all nematodes died.