Western Blot Analysis of GIST-T1 Cells

Western blotting analysis was performed as usually reported [34 (link)]. The GIST-T1 and GIST-T1/IMR cells were challenged with rabbit polyclonal anti-S1P (ab59870), anti-SPK1 (ab260073), anti-β-actin antibodies (ab8826) (Abcam company) and ABCC1 (DF7148) (Affinity company). Proteins were visualized with enhanced chemiluminescence detection reagent according to the manufacturer’s instructions (Pierce, Rockford, Illinois).

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Chen Y., Zhang R., Mi D., Wang Q., Huang T., Dong X., Zhang H., Xiao H, & Shi S. (2022). SPK1/S1P axis confers gastrointestinal stromal tumors (GISTs) resistance of imatinib. Gastric Cancer, 26(1), 26-43.

Publication 2022

ab260073 ab8826 (DF7148

Abcc1 Actin Anti antibodies Cells Chemiluminescence Gist Proteins Rabbit Western blotting analysis

Corresponding Organization :

Other organizations : Chengdu University of Traditional Chinese Medicine, Sichuan Cancer Hospital, University of Electronic Science and Technology of China, Nantong Tumor Hospital

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Variable analysis

independent variables

GIST-T1 and GIST-T1/IMR cell lines challenged with:

dependent variables

Protein expression levels of:

control variables

Protein visualization using enhanced chemiluminescence detection reagent

controls

Positive controls: Not specified
Negative controls: Not specified

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